Protein Misfolding Amyloid Lab Report Example | Topics and Well Written Essays - 3000 words

Protein Misfolding Amyloid - Lab Report Example Fig 3. Fluorescence intensities of a) Tyrosine b) Tryptophan and c) Thioflavin T in Con A , at 40 0C a) b) c) Fig 3 b also shows considerable Trp fluorescence at pH 5.0 and 7.0 while at pH 9.0, intensity remained insignificant again showing buried/ quenched Trp residues. After a lag of 40 h the ThT intensity increased sharply to high level at pH 5.0. At pH 7.0, the plateau was attained after slight increase up to 20 h. At pH 9.0 the intensity increased at 70 h. Amyloid A40 Assay: Fig 4. Fluorescence of a) Tyrosine and b) Thioflavin T in A40 at 250 C Fig 4 a) The ThioflavinT bound to A increases at 40 h decreases thereafter and increases indicating conformational changes. Fig 4 b) Fig 4. shows Tyr intensity decreasing after 60h and so is the Th T intensity however the latter increases thereafter showing increased amyloidal fibril formation but at this time the Tyr seems to be quenched somewhat. Fig 5. TE Micrographs of Con A in pH 5.0 at 0 h Fig 5 a) X10K some amorphous aggregates are already present at this pH Fig 6. TEM showing Con A in pH 5.0 at a) 4 (X40K) and, b) 48h (X40K) c) 48 h at pH 7.0 (X15K and d) 48h at pH 9.0 at 25 0C Fig 6 a) only amorphous aggregates are present Fig 6 b) Fibril formation is clearly visible. Fig 6 c) 48h at pH 7.0 (X15K) Fig 6 d) 48 h at pH 9.0 (X10K) as expected the long amyloid fibrils are in state of formation Fig 7. Con A at 37.2 0C and pH 5.0 after 24h The physiological temperature and low pH shows abundant short rods Fig 8. A40 at 25 0 C a) 0, and b) 3h. (X20K) neither shows fibrils despite positive ThT fluorescence. Fig 7 shows AB40 as small...But at pH 9.0 the intensity decreased sharply from beginning up to 70h and then attained a plateau. The decrease of 400u indicated huge conformational change leading to buried and /or quenched Trp residues. Th T fluorescence decreased slightly up to 50 hrs and increased sharply thereafter reaching at peak at 80 hrs and then decreasing considerably. At pH 7 the intensity increased from beginning, reaching a max at 50h and decreasing sharply, thereafter. The Th T fluorescence shows reversible trend in these experiments and conformational changes are occurring fast. At pH 9.0, there was considerable increase in fluorescence intensity after 75h showing fibrillation (fig 1 c). Highest intensity was observed at pH 5.0 while considerably high Intensity at pH 7.0. However at pH 9.0 there was negligible intensity for tyrosine. The latter temperature and pH combination either create conditions for Tyrosine quenching or the aggregates deeply bury this amino acid as a result of conformational changes (Fig 3a). Fig 4. shows Tyr intensity decreasing after 60h and so is the Th T intensity however the latter increases thereafter showing increased amyloidal fibril formation but at this time the Tyr seems to be quenched somewhat. Only large amorphous structures seen after 72 h while a solitary long fibril is seen in the upper region after 96h (Fig 8 b).

Roles and Function Paper Essay Example | Topics and Well Written Essays - 500 words - 1

Roles and Function Paper - Essay Example Planning for emergencies is also a management function. Organizing the health care services and staff is another important management function in health care setting. Leading the staff is another function of management where meeting need to be held with staff to give them instruction and direction. Controlling is the final function where the team of staff needs to be built and maintained. Controlling risky and emergency situation is also a function of management. Such functions are highly useful in managing me in my previous job. I have planned my working schedule well once I was working. I have planned how to meet the patient’s need and the goals of my employer. I was well prepared for uncertain situations. I was well organized in my work and I used to follow my work schedule and requirement properly. Also my co – ordination with staff and patients was good because I thought it made my profession efficient. Controlling my behavior, emotions and attitude was more of benefit for me while working. The health care function manager needs to perform many functions. The most important function of a health care manager is maintaining the smooth operation of the health care system of the organization. Like any other business, health care sector need good management to run it smoothly. Planning, directing, coordinating, supervising are all needed for an effective running of a health care service centre. In a diversifying health care sector, there may be employees from different backgrounds and co – iodinating and leading them is not easy. According to( Lavey,2007,pg.363-74) â€Å"The natural population growth, aging of the population and consumer revolution have all increased the demand for health services in public hospitals†. Specialized skills and knowledge is essential in diversified health care sector. These are some information analyzed from the class. This class is of